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When I have this problem I always put the one I'm not sure of with the boys, that way you end up with only one accidental litter. Of course you could just put that one in a separate tank if you have the room. I've had this happen after I'd carefully sexed a liter, and I always wonder if my mind is going...long haired litters are the most difficult in this regard as if you don't sex them accurately before they get a full coat, it's often just a guessing game.